Write the principle of electrophoresis. Explain Isoelectric focusing using a neatly label diagram. List its advantages.

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Electrophoresis is a separation technique that is based on the principle of movement of charged molecules under the influence of an electric field. Proteins, being amphoteric in nature, carry both positive and negative charges depending on the pH of the medium. When an electric field is applied, the net charge on a protein determines the direction and rate of its migration. The mobility of a molecule depends on its charge-to-mass ratio, shape, and the strength of the electric field. _____________ _____ _____________ _________ __________ __________ _______ _______ _____ ___________ _________ ______ ____________ ___________.
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Degree : BACHELOR DEGREE PROGRAMMES
Course Name : B.Sc. Honours in Biochemistry
Course Code : BSCBCH
Subject Name : Proteins
Subject Code : BBCCT 105
Year : 2023